Confocal Imaging on Microprojectile-bombarded Pollen of Gossypium hirsutum L.
Oct 8, 2018

Biotechnology WCRC WCRC2
Confocal laser scanning microscopy (CLSM) was introduced to study transgenic molecular
cytobiology. Scanning electron microscopy (SEM) was used to observe the transferring traces
of accelerated, DNA-coated, metal particles labeled with 4,6-diamidino-2-phenylindole
(DAPI) in mature cotton pollen (G. hirsutum L.). The results indicated that pollen exine can
be penetrated by the high-velocity microprojectiles, and the microprojectile with fluorescent
probe and its transferring trace in the exine-detached pollen can be observed. The optical
sectioning imaging analysis indicated that the microprojectile can be introduced into a
bombarded pollen grain approximately 20.6 m deep. In bombarded grains, transient
expression of a β-glucuronidase (GUS) gene in pBI121 was certified by histochemical assay.

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