Glycinebetaine Treatment Improves Cotton Yields in Field Trials in Pakistan
Apr 26, 2019
This study was designed to test the effectiveness of glycinebetaine (an osmoprotectant purified from sugar beet and applied as a spray of ‘Greenstim’) in improving the yields of cotton under normal cultivation conditions in the Punjab of Pakistan. Field trials were conducted in replicated small plots at two sites over two years to determine the optimal timing and dose of glycinebetaine. CIM-240 was sown in 1996 at the Postgraduate Agricultural Research Station (PARS), Faisalabad but was badly affected by whitefly and cotton leaf curl virus. CIM-443 was sown in 1996 and 1997 at the Central Cotton Research Institute (CCRI), Multan. This was resistant to leaf curl virus, and was sown at PARS in 1997. Generally, glycinebetaine concentrations and time of application had little effect on physiological parameters. In 1996, glycinebetaine concentrations were significantly increased in the trial at Faisalabad, but not at Multan. In 1996 the highest yield of seed cotton at Faisalabad (136 % of the control) was obtained with 3-kg/ha glycinebetaine applied at squaring. At Multan, seed cotton yields were highest with 3 and 6 kg/ha glycinebetaine applied at squaring. Glycinebetaine dose and time of application had highly significant (P<0.001) effects on yield, total dry matter and bolls per plant. Glycinebetaine-treated plants also had more nodes at maturity than the controls. Mean boll weight, % flower and boll shedding were not affected. In 1997 the highest yields were obtained when glycinebetaine was applied at first flowering at PARS, but at the pre-squaring stage at CCRI.