Isolation and characterization of the GbVIP1gene and response to Verticillium wilt in cotton and tobacco
Mar 11, 2019

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Isolation and characterization of the GbVIP1gene and response to Verticillium wilt in cotton and tobacco





  • ZHANG Kai ,

  • ZHAO Pei ,

  • WANG Hongmei Email author,

  • ZHAO Yunlei ,

  • CHEN Wei ,

  • GONG Haiyan ,

  • SANG Xiaohui  and

  • CUI Yanli 



Contributed equally





Journal of Cotton Research20192:2

https://doi.org/10.1186/s42397-019-0019-0








Abstract




Background


Verticillium wilt is a serious soil-borne vascular disease that causes major losses to upland cotton (Gossypium hirutum L.) worldwidely every year. The protein VIP1 (VirE2 interaction protein 1), a bZIP transcription factor, is involved in plant response to many stress conditions, especially pathogenic bacteria. However, its roles in cotton response to Verticillium wilt are poorly understood.





Results


The GbVIP1 gene was cloned from resistant sea-island cotton (G. barbadense) cv. Hai 7124. Expression of GbVIP1 was up-regulated by inoculation with Verticillium dahliae and exogenous treatment with ethylene. Results of virus-induced gene silencing suggested that silencing of GbVIP1 weakened cotton resistance to Verticillium wilt. The heterologous expression of GbVIP1 in tobacco showed enhanced resistance to Verticillium wilt. The PR1, PR1-like and HSP70 genes were up-regulated in GbVIP1 transgenic tobacco after Verticillium wilt infection.





Conclusion


Our results suggested that GbVIP1 increased plant resistance to Verticillium wilt through up-regulating expressions of PR1, PR1-like, and HSP70. These results provide new approaches to improving resistance to Verticillium wilt in upland cotton and also have great potential for disease-resistance breeding of cotton.






Keywords




  • Cotton

  • VIP1

  • Verticillium wilt




https://jcottonres.biomedcentral.com/articles/10.1186/s42397-019-0019-0
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