A plant expression vector, pBinLK, carrying two insecticidal genes: pea lectin gene and soybean Kunitz trypsin inhibitor gene, were constructed and successfully transferred into 4 upland cotton (Gossypium hirsutum L.) cultivars, Xinluzao-1, Xinluzhong-2, Jihe-321 and Liao-9 via Agrobacterium-mediated transformation. Hypocotyl segments from aseptic seedlings were used as transgene recipients. After co-cultivation, kanamycin-resistant calli were screened, and somatic embryos and regenerated plants were obtained using various media. Transgenic cotton plants with two insecticidal genes were confirmed by ELISA, PCR and PCR Southern analysis. Bioassays demonstrated that the transgenic plants had significant resistance to larvae of cotton bollworm (Helicoverpa armigera Hübner).