Resistance of cotton cultivars in the Sudan to Xanthomonas campestris pv. malvacearum conferred by B2 B6 gene combination is no longer effective after the appearance of new races of the pathogen. Thus, new sources of resistance or new gene combinations are needed to protect the crop. This study was carried out to identify such a new source or gene combination to confer resistance to the prevailing races in the Sudan. Highly resistant plants from some F2 plants were recovered. The S295 cotton cultivar (B12) showed high resistance to post-Barakat race. Some MAR cotton germplasm (MAR 5 & 6) was also showed high resistance to this race. This high resistance was also observed in the F4 segregating populations of the introduced crosses E1043 × B2 B6 and B2 B6 × Pima 32.