Sucrosyl Oligosaccharide Metabolism and Cotton Fiber Development
Apr 19, 2019
The object of this study was to determine which oligosaccharides correlate with cell wall synthesis in developing cotton fibers. This study employed bolls taken from sequential fruiting branches on the same plant to rule out environmental and plant to plant differences. Cold aqueous extracts of developing cotton fibers have been analyzed by HPAEC-PAD (Murray, 1998). Sucrosyl oligosaccharides appear to function as cell wall precursors. A decrease in sucrose concentration and a concomitant increase in raffinose concentration characterize maximal secondary wall synthesis in cotton fibers. We have confirmed the identification of raffinose, stachyose, verbascose and tentatively identified ajugose and other sugars in the series. Environmental conditions appear to influence the concentrations of glycerol, m-inositol, galactinol, melibiose, and manninotriose in developing cotton fibers. The relative concentrations of sucrosyl oligosaccharides were found to vary fibers from plants subjected to high stress in irrigation experiments. Differences in glycoconjugate profiles in the fibers are apparent in irrigation experiments several days before significant differences are apparent in leaf water potentials. Differences in glycoconjugate profiles are also apparent in fibers from bolls collected at 7am, noon and 7pm suggestive of a diurnal rhythm. This diurnal variation is of interest since the major portion of cell wall deposition occurs at night. (Murray, A. K., 1998, U.S. Patent No. 5,710,047).